| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115097 |
| Chromosome: | chromosome 10 |
| Location: | 810206 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423300 | (1 of 1) 3.4.22.40 - Bleomycin hydrolase / Aminopeptidase C (Lactococcus lactis) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCATCGGAGCGAGCGGAGCCTAGCTTG |
| Internal bar code: | GCTGTGTGTAGGCAACATGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 62.0408 |
| LEAP-Seq n confirming: | 3800 |
| LEAP-Seq n nonconfirming: | 2325 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCGGAAATGAATTACAGG |
| Suggested primer 2: | AGACACGCGTTCTGACACAC |