| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.115100 |
| Chromosome: | chromosome 5 |
| Location: | 562452 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCTGCTTCCCTGCTGGCGTTGTTTTTG |
| Internal bar code: | AAGAGCGTACGTCTGCGTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 67.344 |
| LEAP-Seq n confirming: | 1425 |
| LEAP-Seq n nonconfirming: | 691 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGTCAAGTGGAGACGGG |
| Suggested primer 2: | CATGTGAATGTTTCACCCCA |