Insertion junction: LMJ.RY0402.115116_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.115116
Chromosome:chromosome_9
Location:3655261
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g390541 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TACTGCAATGCAGTGATGATAGTGGCTTTC
Internal bar code:TCGTTCGCCACATCTCGACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:531
LEAP-Seq percent confirming:98.6013
LEAP-Seq n confirming:32851
LEAP-Seq n nonconfirming:466
LEAP-Seq n unique pos:99

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATGGAGACGGTAGAGTG
Suggested primer 2:TACAACTGCAGGCATCAAGC