| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.115119 |
| Chromosome: | chromosome 4 |
| Location: | 3918001 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g230830 | (1 of 1) IPR000104//IPR001012//IPR029071 - Antifreeze protein, type I // UBX domain // Ubiquitin-related domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGGAGCGAGGAGCTGGCTGGAAGGTG |
| Internal bar code: | CCCCCAATCTTGCCAGTCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 99.8085 |
| LEAP-Seq n confirming: | 5733 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCATGCTGCTCTGTGACT |
| Suggested primer 2: | ATGAGGGACGCCTAGAGACA |