Insertion junction: LMJ.RY0402.115127_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.115127
Chromosome:chromosome 3
Location:6741409
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g198000 PPP15 (1 of 2) PF00481//PF13672 - Protein phosphatase 2C (PP2C) // Protein phosphatase 2C (PP2C_2); Phosphoprotein phosphatase 2C-related intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCATTGCAGGCACATGGGGTTGCATGAAC
Internal bar code:CCAACCGGTCACTGATTTCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1047
LEAP-Seq percent confirming:99.2213
LEAP-Seq n confirming:1529
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCTAGGACACGTTCACAT
Suggested primer 2:TCTTCTTGACGCACACGAAC