| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.115133 |
| Chromosome: | chromosome 4 |
| Location: | 2051830 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219050 | SRR10 | Scavenger receptor cysteine rich (SRCR) protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGACCCACTCCCATGCCGTATCACTTG |
| Internal bar code: | ATGAGCGGCATTGCAACTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 98.5916 |
| LEAP-Seq n confirming: | 280 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAATCCAAACCTCAGCCG |
| Suggested primer 2: | TCTCAATGAGTTCTGTGCCG |