Insertion junction: LMJ.RY0402.115146_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.115146
Chromosome:chromosome 8
Location:4242434
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g380650 GT90-13,GT90F13 GT90 family protein 13; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTCCACCCTCCGCCCTTCCACGTTTTCC
Internal bar code:TCCCGAATCCACGAGCTTTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:571
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGCACGCTACCTAAGC
Suggested primer 2:AACACCCTGTCCTTCATTCG