| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.115174 |
| Chromosome: | chromosome 9 |
| Location: | 1245301 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399550 | (1 of 1) PTHR10857//PTHR10857:SF11 - COPINE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGACAAGACCCCGTCTCCCGCAGCCAAA |
| Internal bar code: | ACCGCGCTGCTCCGCAATGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 469 |
| LEAP-Seq percent confirming: | 96.8987 |
| LEAP-Seq n confirming: | 4343 |
| LEAP-Seq n nonconfirming: | 139 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTCCTTCACTTGACTTC |
| Suggested primer 2: | AATGGGAAGTGCATAAACGC |