| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.115183 |
| Chromosome: | chromosome 2 |
| Location: | 3896563 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096200 | (1 of 1) K06990 - uncharacterized protein (K06990) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCAATCCCTATGCGACCCCCACCGTCGC |
| Internal bar code: | GGGTAACGAGCGGGGGCGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 890 |
| LEAP-Seq percent confirming: | 37.0474 |
| LEAP-Seq n confirming: | 1453 |
| LEAP-Seq n nonconfirming: | 2469 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCATGTCTTCCTCAAGC |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |