| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.115210 |
| Chromosome: | chromosome 15 |
| Location: | 720240 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640651 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCCGGCACGCCAGGCTCTCCTCCACCA |
| Internal bar code: | TCAGTGTATTATGGCATTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 99.6596 |
| LEAP-Seq n confirming: | 1171 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGTGGGAAAGAAGGAG |
| Suggested primer 2: | ATCGCATTGGTTCAAACTCC |