| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115245 |
| Chromosome: | chromosome 6 |
| Location: | 6453949 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292750 | (1 of 2) PF05694 - 56kDa selenium binding protein (SBP56) (SBP56) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCGTCAGTAAAGGCCTCACGTACAGAC |
| Internal bar code: | AGGGACGGCGTCCGTCCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 85.9485 |
| LEAP-Seq n confirming: | 367 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTATGGGCAGGTCGTGAAC |
| Suggested primer 2: | GTGGGCGAGTCTTGGTATGT |