| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115269 |
| Chromosome: | chromosome 10 |
| Location: | 2007223 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432550 | KCN2 | Six-transmembrane domain potassium channel alpha subunit; (1 of 1) PTHR11537:SF133 - PROTEIN EXP-2, ISOFORM C | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGACGTTGCCGATTCAAGAGCGGAAGCT |
| Internal bar code: | CGAACGCGCCGGTAGAGCCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 965 |
| LEAP-Seq percent confirming: | 99.0931 |
| LEAP-Seq n confirming: | 2841 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCTCGAGTTTAACAGGG |
| Suggested primer 2: | GCCATGCTTGTATGCAGCTA |