| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115271 |
| Chromosome: | chromosome 6 |
| Location: | 5156297 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281400 | NAH1 | Cation antiporter; (1 of 11) PF00999 - Sodium/hydrogen exchanger family (Na_H_Exchanger) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGTAAATGAGATGCCTTAGCAGGTGTG |
| Internal bar code: | CACGCGGAAATGCACAGCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 719 |
| LEAP-Seq percent confirming: | 98.8469 |
| LEAP-Seq n confirming: | 4629 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTCTCCATCATCATCCA |
| Suggested primer 2: | TTACAACAGCAGCTTGGTGG |