| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.115282 |
| Chromosome: | chromosome 6 |
| Location: | 8322269 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306150 | (1 of 1) IPR000104//IPR004919 - Antifreeze protein, type I // Domain of unknown function DUF262 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCCGGCCTTGCCATGCATGGCTGGGCG |
| Internal bar code: | CTGTCTCCGCCGCCGTGCAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 272 |
| LEAP-Seq percent confirming: | 99.8599 |
| LEAP-Seq n confirming: | 1426 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCACCGGGTGTTCACATA |
| Suggested primer 2: | GCTTTGCATACGTCCCATTT |