Insertion junction: LMJ.RY0402.115309_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.115309
Chromosome:chromosome 12
Location:2061551
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g509700 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTTTCCTCATTTGTCCCTTGTGACTCAC
Internal bar code:GGAGAACACTTTGTCTGGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:750
LEAP-Seq percent confirming:99.5516
LEAP-Seq n confirming:222
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCACGCACAACCAACATC
Suggested primer 2:CGCCTTTCCACAAGTCTCTC