| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.115329 |
| Chromosome: | chromosome 16 |
| Location: | 5347204 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681850 | (1 of 2) 2.4.2.9//2.7.1.48 - Uracil phosphoribosyltransferase / UMP pyrophosphorylase // Uridine kinase / Uridine monophosphokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCTAAGTCATCCGGTGTGGCATGCGGC |
| Internal bar code: | TTCCTAATAGATTCTGTAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1183 |
| LEAP-Seq percent confirming: | 99.7032 |
| LEAP-Seq n confirming: | 3695 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACAGTCAGTTTGGGGTC |
| Suggested primer 2: | CGTACAATGGCCTTCACCTT |