| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.115354 |
| Chromosome: | chromosome 7 |
| Location: | 5549123 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351450 | (1 of 1) PTHR14030:SF4 - MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 BETA | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACGGAGTATGTGGGGCTGCGCCCCGCC |
| Internal bar code: | AACAAAGTCCCCAGATGAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 867 |
| LEAP-Seq percent confirming: | 71.381 |
| LEAP-Seq n confirming: | 429 |
| LEAP-Seq n nonconfirming: | 172 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCAGTGACGCTCTACCG |
| Suggested primer 2: | GCCTCACTCTCAAAACTGCC |