Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.115360 |
Chromosome: | chromosome_9 |
Location: | 283054 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g392867 | FMG1-B | Flagella membrane glycoprotein, major form | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGCGATGGCCTTGGCGGACACAATGCTCTG |
Internal bar code: | AAACCCCGACTTCGTGGGATGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 682 |
LEAP-Seq percent confirming: | 97.3811 |
LEAP-Seq n confirming: | 2417 |
LEAP-Seq n nonconfirming: | 65 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTGATGGTCAGGAGGGTGT |
Suggested primer 2: | CGTCGTAATCTACCCCTCCA |