| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115367 |
| Chromosome: | chromosome 10 |
| Location: | 2197716 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTCTCGCCGTAAGACGCCCACAAAAGG |
| Internal bar code: | ATACCAGTCTACCGGCTTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 99.8688 |
| LEAP-Seq n confirming: | 761 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAGTCATGTCAACCGTGT |
| Suggested primer 2: | CGGCAGCTGTAGCAAGTTAG |