Insertion junction: LMJ.RY0402.115408_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.115408
Chromosome:chromosome 15
Location:1725595
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g643703 RLS9 (1 of 89) PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) (RRM_1); RegA/Rls-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCATCTGCACGTGCGAAGAGGGAAGGGG
Internal bar code:CTTGTTGGCTGGTGGGGTGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:58
LEAP-Seq percent confirming:97.9592
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCCCACACACCTATTACT
Suggested primer 2:ACCGGTGAAGAAATACGCAC