| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.115411 |
| Chromosome: | chromosome 14 |
| Location: | 1776663 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619850 | ELG36 | (1 of 2) 2.7.7.52 - RNA uridylyltransferase / TUT; Exostosin-like membrane protein, a heparan sulfate glycosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTAGGGCCAGCTGTTTTCAACATCACAG |
| Internal bar code: | GAATACTAGGGATTCTTCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 99.4349 |
| LEAP-Seq n confirming: | 7390 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGCTTTTGGTTTGCTTG |
| Suggested primer 2: | CAACGCCAGCACTTGATTTA |