Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.115456 |
Chromosome: | chromosome_12 |
Location: | 5792413 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g533351 | CLPB5 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGATATGCGTGAGTGCAGAGATTGTGTGTG |
Internal bar code: | AATATCAATATAGTGCGAATCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 121 |
LEAP-Seq percent confirming: | 64.3341 |
LEAP-Seq n confirming: | 285 |
LEAP-Seq n nonconfirming: | 158 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCTGTTCAGAGCCTGGTAG |
Suggested primer 2: | TTAATACACTCCCCCTCCCC |