Insertion junction: LMJ.RY0402.115465_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.115465
Chromosome:chromosome 5
Location:3441306
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g241350 (1 of 1) 3.6.3.36 - Taurine-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTGTAGCCAGGGGGGGGCTGGCTTGCCG
Internal bar code:TGTTTCTTTAAACAATACGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:396
LEAP-Seq percent confirming:98.8588
LEAP-Seq n confirming:693
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAGAACCTGGAGTACAGC
Suggested primer 2:AGCGTGTGGACACTAATCCC