| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115489 |
| Chromosome: | chromosome 3 |
| Location: | 7488046 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205550 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGGATGTGGGCATGAACGTGCGCCGCA |
| Internal bar code: | TTGGGCGGCTGCGCAGGCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 429 |
| LEAP-Seq percent confirming: | 97.9241 |
| LEAP-Seq n confirming: | 4670 |
| LEAP-Seq n nonconfirming: | 99 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAAGGTGAGCGTGTAGT |
| Suggested primer 2: | TTTATGGCCGCCTGACTAAC |