| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115554 |
| Chromosome: | chromosome 6 |
| Location: | 1260666 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258100 | (1 of 2) PTHR12436//PTHR12436:SF4 - 80 KDA MCM3-ASSOCIATED PROTEIN // LEUKOCYTE RECEPTOR CLUSTER MEMBER 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGCGCTGTTGCCCCGCTCCAGATGTGT |
| Internal bar code: | CGCGGCACATGGTTGGGTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 977 |
| LEAP-Seq percent confirming: | 96.2939 |
| LEAP-Seq n confirming: | 10471 |
| LEAP-Seq n nonconfirming: | 403 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATGTCCCAGACATAGGC |
| Suggested primer 2: | TCTGAACACAGCATTGCACA |