| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115557 |
| Chromosome: | chromosome 16 |
| Location: | 1845762 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGTAGCTGCGCTCGCTCTATGGCCCGA |
| Internal bar code: | CGCGGTTGCGACGAATACGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 856 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 1760 |
| LEAP-Seq n nonconfirming: | 160 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCAAGGGGTGACAAGAT |
| Suggested primer 2: | GCTTGTGTTGTTGGTGTTGG |