| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.115620 |
| Chromosome: | chromosome 14 |
| Location: | 1144811 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615550 | TGL19 | Putative triacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTCAGCTCCATATACATGGCTGTGTATG |
| Internal bar code: | AACTGGAACTTAGGATATCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 99.375 |
| LEAP-Seq n confirming: | 318 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCGGGTTGGTGAGATAC |
| Suggested primer 2: | ACACCCTACGCATGCCTTAC |