Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.115627 |
Chromosome: | chromosome_2 |
Location: | 5339030 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g107050 | DHC5 | Dynein heavy chain 5, inner arm dynein b (monomeric) | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCTTGAGTGTCTGCACAAACTTGGGATCC |
Internal bar code: | GGGAACAGTGGGCCGAAATAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 742 |
LEAP-Seq percent confirming: | 99.7238 |
LEAP-Seq n confirming: | 1444 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGAGCAGCCACATTTCTGA |
Suggested primer 2: | GTTACACACCAACCGTGTGC |