Insertion junction: LMJ.RY0402.115630_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.115630
Chromosome:chromosome 16
Location:3807015
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g689550 PTK8 Putative tyrosine kinase; (1 of 10) PTHR23257//PTHR23257:SF520 - SERINE-THREONINE PROTEIN KINASE // IP11267P CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TGGCTCGCCGAACCTGCAGACCAGAGATGC
Internal bar code:AAGTGAATCAGGTACAGACGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:384
LEAP-Seq percent confirming:99.6835
LEAP-Seq n confirming:315
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGTCGGCTAACACACCTG
Suggested primer 2:GATGAGACGCAAGGGAAGAG