| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.115633 |
| Chromosome: | chromosome 3 |
| Location: | 4302619 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174700 | (1 of 1) 3.1.22.1 - Deoxyribonuclease II / Lysosomal DNase II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAGCATGGGCCTGCTTGCCGCCTCGGC |
| Internal bar code: | TGGACTATAGTGACGGTTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 195 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTACGGCAGCTCATTGAC |
| Suggested primer 2: | CACTGCCTCTCTCACGATCA |