| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.115640 |
| Chromosome: | chromosome 6 |
| Location: | 7171744 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297750 | SPL3 | SF3A3 splicing factor 3a, subunit 3; (1 of 1) K12827 - splicing factor 3A subunit 3 (SF3A3, SAP61, PRP9) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAATTATGGGGGCGCGCCGGCTGCCACA |
| Internal bar code: | ATATATGGACGTGATAAAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 686 |
| LEAP-Seq percent confirming: | 99.6026 |
| LEAP-Seq n confirming: | 2256 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAATGTAGGGCCATAAGA |
| Suggested primer 2: | GAGCTGACTCCTGGATCTGG |