| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.115640 |
| Chromosome: | chromosome 6 |
| Location: | 7171750 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGTTATCACGACGCATACGCTAAGACAT |
| Internal bar code: | AAAACTACATCTCGGGGCATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 99.3691 |
| LEAP-Seq n confirming: | 630 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAATGTAGGGCCATAAGA |
| Suggested primer 2: | GAGCTGACTCCTGGATCTGG |