| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.115648 |
| Chromosome: | chromosome 3 |
| Location: | 5514483 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185950 | (1 of 1) IPR006642//IPR026319 - Zinc finger, Rad18-type putative // Zinc finger C2HC domain-containing protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATATACGCGCGATCTCCCAATCCCAGTC |
| Internal bar code: | CAGCGTTTGGTCAGTTTTCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 516 |
| LEAP-Seq percent confirming: | 75.3219 |
| LEAP-Seq n confirming: | 1053 |
| LEAP-Seq n nonconfirming: | 345 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTAACCCCTCCACCTCC |
| Suggested primer 2: | GATGCGTACAGAGTGGACGA |