| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115648 |
| Chromosome: | chromosome 10 |
| Location: | 4523400 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452150 | (1 of 100) IPR001810 - F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCGCGCTCCTGTGGACACACCCCATAC |
| Internal bar code: | ACGTGGAGGAAGGTCGGCTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 99.7641 |
| LEAP-Seq n confirming: | 5498 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGCAAAGAGCAGGACTA |
| Suggested primer 2: | TTGTACTTTCTGCCGTTCCC |