| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.115688 |
| Chromosome: | chromosome 10 |
| Location: | 2131156 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433476 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGCGTGCATAGACTGCATACGTGCACA |
| Internal bar code: | AAAAACAGAGGCGCGTCAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 87.9678 |
| LEAP-Seq n confirming: | 1528 |
| LEAP-Seq n nonconfirming: | 209 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCTCACAGCCCTACTTC |
| Suggested primer 2: | CTAGCTAGCGCACAAATCCC |