| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.115788 |
| Chromosome: | chromosome 12 |
| Location: | 8246498 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550350 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGAATGTAACCTCCCCCCTGTGCTCCG |
| Internal bar code: | TCCGGGTGGTCGGTCGGCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 96.25 |
| LEAP-Seq n confirming: | 154 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGTGGATGTGATACTGG |
| Suggested primer 2: | ATGACACGACCTGTGCTGTG |