Insertion junction: LMJ.RY0402.115850_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.115850
Chromosome:chromosome 13
Location:4507876
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g602450 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTGGAAGGGGCGGCAGCGCGCTAACAG
Internal bar code:GTACCAGCTGACCTCCCTCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:743
LEAP-Seq percent confirming:99.8016
LEAP-Seq n confirming:2012
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCAAACCACAGCACAATG
Suggested primer 2:TCTCACATCCAACAAGCTGC