| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115852 |
| Chromosome: | chromosome 3 |
| Location: | 4629374 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177300 | HAP4 | (1 of 3) 1.11.1.10 - Chloride peroxidase / Vanadium haloperoxidase; Haloperoxidase-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGCAGAAGCGCATTGGTGACAATTTCA |
| Internal bar code: | AATTTACGCCAACCGCGCTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 98.7455 |
| LEAP-Seq n confirming: | 2755 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCTACTGTCATCGCCA |
| Suggested primer 2: | GCTCCCGGTACTTGATACGA |