| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115955 |
| Chromosome: | chromosome 5 |
| Location: | 823131 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248050 | (1 of 1) IPR012889 - L-fucose isomerase, N-terminal-2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGACCGGGAGGTGCTGAGGCTGGTGGC |
| Internal bar code: | TAGCATGCGCGGCGGAACAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 84 |
| LEAP-Seq percent confirming: | 71.6867 |
| LEAP-Seq n confirming: | 119 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCGGTTGCACTACACTAT |
| Suggested primer 2: | TTCCTGCACTACCGTTACCC |