| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.115956 |
| Chromosome: | chromosome_13 |
| Location: | 3134400 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g585050 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCAAGATAGAATAATTGGGGGCAGGTGGG |
| Internal bar code: | CCTTTCGGCCAAGGCAGCTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 99.4113 |
| LEAP-Seq n confirming: | 1182 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAGTGAGGCGAGGAGCAT |
| Suggested primer 2: | TAGGGGGTACAGATGAAGCG |