| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.115964 |
| Chromosome: | chromosome 9 |
| Location: | 5841789 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402256 | (1 of 3) 3.4.11.1 - Leucyl aminopeptidase / Peptidase S | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCCTGGCTACTGAAGCCGGCCGCCCTG |
| Internal bar code: | GTGAACCGAGGGATACCGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 69.1697 |
| LEAP-Seq n confirming: | 958 |
| LEAP-Seq n nonconfirming: | 427 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGGCGTGTATCAAGGAT |
| Suggested primer 2: | TTAACGGGTGGGAAATTGAA |