Insertion junction: LMJ.RY0402.116013_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.116013
Chromosome:chromosome 11
Location:1200492
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467692 (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCACCAGCCGCATTTGCGGCATTTCATG
Internal bar code:GCAGTCGAGGATACAGTCGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:489
LEAP-Seq percent confirming:99.0862
LEAP-Seq n confirming:759
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GACTTGCTCCTGTCTACGCC
Suggested primer 2:AAGTACAGAGGCCCTGCAAA