| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116019 |
| Chromosome: | chromosome 14 |
| Location: | 2980781 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627917 | (1 of 4) 3.1.2.2//3.1.2.20 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase // Acyl-CoA hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCGCACGCCCCCAAGCAACTGGTTTCA |
| Internal bar code: | ATGGCAACTATGGTTTTGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 715 |
| LEAP-Seq percent confirming: | 99.1918 |
| LEAP-Seq n confirming: | 4541 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGCACTCTATCCAATGA |
| Suggested primer 2: | TACACGGTGTGTGTGTGTGG |