Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.116023 |
Chromosome: | chromosome 13 |
Location: | 2274542 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g578900 | MOT27 | (1 of 98) IPR023214 - HAD-like domain | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTTAACCCCCACCTCACTATCCTTGGCT |
Internal bar code: | AAGTAAACTCTGTGCTGTCACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 289 |
LEAP-Seq percent confirming: | 79.213 |
LEAP-Seq n confirming: | 1852 |
LEAP-Seq n nonconfirming: | 486 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAAAGTAGTGGTAGGGGCA |
Suggested primer 2: | CTTTCCCCTCTCCCCACTAC |