| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.116023 |
| Chromosome: | chromosome 13 |
| Location: | 2274548 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578900 | MOT27 | (1 of 98) IPR023214 - HAD-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATAAGTAAGGTAACCGTAAGGCGCTGCAA |
| Internal bar code: | CGCGTGGCAATACTTAGCATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 97.7524 |
| LEAP-Seq n confirming: | 2827 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAAGTAGTGGTAGGGGCA |
| Suggested primer 2: | CTTTCCCCTCTCCCCACTAC |