Insertion junction: LMJ.RY0402.116049_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.116049
Chromosome:chromosome 14
Location:532946
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g611484 (1 of 4) IPR023485 - Phosphotyrosine protein phosphatase I superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGTGGTTGCCTGGCAGCTCGTCCACCG
Internal bar code:TGGTTTACAGAACTGCTGGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:984
LEAP-Seq percent confirming:99.6712
LEAP-Seq n confirming:5457
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGCATAAAGGCTTATCTC
Suggested primer 2:CAGCTGTGACCCTCATCAGA