Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.116077 |
Chromosome: | chromosome_11 |
Location: | 2623952 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g476376 | FAP221 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AATGGAGTTGTAGCGGTAAAGGAGGGGTTT |
Internal bar code: | TTAATGGCCACCTCTTGGCACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 209 |
LEAP-Seq percent confirming: | 99.5964 |
LEAP-Seq n confirming: | 3208 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGCATTCGCAGTAAGGACA |
Suggested primer 2: | TACAGGTGCAGAGCGAATTG |