| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.116085 |
| Chromosome: | chromosome_12 |
| Location: | 5789594 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g533351 | CLPB5 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGGTCTTTTTGAACTTGTCGGTGCTGCTG |
| Internal bar code: | CAGAATTGAGAGGGCCCTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 350 |
| LEAP-Seq percent confirming: | 83.9023 |
| LEAP-Seq n confirming: | 12535 |
| LEAP-Seq n nonconfirming: | 2405 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGTCTCCACCCTATCCA |
| Suggested primer 2: | CGCTGTAATTCACTCTGCCA |