| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116115 |
| Chromosome: | chromosome 13 |
| Location: | 3652224 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588736 | (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGTGTTGCGGTGCAGGGCGGCATGTTC |
| Internal bar code: | TGGCGCCGCCGTGAGACATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 99.5146 |
| LEAP-Seq n confirming: | 1230 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCTGCACCCTACAGGTGG |
| Suggested primer 2: | ACAACAACAGCAGCAGCAAC |