| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116133 |
| Chromosome: | chromosome 11 |
| Location: | 807301 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467641 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCATCATTTACCTCGCGCAGATCTCCAA |
| Internal bar code: | CGGCACCTGTAGCCATACCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 694 |
| LEAP-Seq percent confirming: | 99.7066 |
| LEAP-Seq n confirming: | 1699 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTCTCCGTCTGGCAAGC |
| Suggested primer 2: | CGACAGTTGTTCGATGGATG |